PCR targeting Omp31, IS711, and BCSS confirmed this isolate as B. DNA was extracted from the isolate to amplify the Brucella-specific genes ( 16S rRNA, BCSP31, and Omp2). The average nucleotide identity (ANI) of strain. After several passages through the Brucella agar plate, the pure culture product (the isolate) tested negative for H 2S production and positive for oxidase and urease. It exhibits antimicrobial activity assembled using CLC Genomics Workbench 7.5 (CLC bio, Aarhus, Denmark).
#Clc genomics workbench 7.5 software#
Of course, if this software is too old, as it appears, it is. And if it is a licenced version, the company could help you. After a blood culture, the cultivated product was streaked on both a blood agar plate and a Brucella agar plate and incubated at 37℃ and 5% CO 2 atmosphere. version 7.5.2 should work with your software. melitensis was further confirmed at the Division of Zoonoses at the Korea Centers for Disease Control and Prevention after the approval of the Institutional Review Board of Konkuk University Medical Center, Seoul, Korea. Therefore, the identification result of B. This is the first report of MWMV in Turkey.The Vitek 2 system database has a limitation in identifying Brucella spp.: it misidentifies B. MW362132), which revealed 99.15% sequence identity with two MWMV Greek isolates (LN810061 and KF772944) by BlastN analysis. Then it was submitted to GenBank (Accession No. The raw sequence was manually refined and obtained trimmed sequence (589 bp) was analyzed by CLC Genomics Workbench v.7.5 software (QIAGEN, Denmark). One of the randomly selected positive PCR products was sent for sequencing (REFGEN Biotechnology, Turkey). The MWMV-infected plants showed mild to severe mosaic, blistering, and mottling on the leaves. RT-PCR results confirmed DAS-ELISA results of the six MWMV-positive samples. This technology is available in three editions, Free, Commercial, and Enterprise. The workbench supports and integrates into a typical NGS workflow. 2005) and RT-PCR tests were performed using specific primers MWMV-5′/MWMV-3′ (5′-AGCAAGCGCCATACTCTGA-3′ and: 5′-CAAACTCCA TTAACATTCGG-3′), to amplify partial sequence of polymerase (NIb) and coat protein (CP) genes, about 627 bp (Lecoq et al. Description: CLC Genomics Workbench is a software application for the analysis and visualization of data from all major next-generation sequencing (NGS) platforms. To confirm the DAS-ELISA results, total RNA extractions were obtained by using silica-gel based method (Foissac et al. According to the results of DAS-ELISA, six MWMV-infected samples (13.33%) were determined. Bioz Stars score: 86/100, based on 1 PubMed citations. For determination of MWMV infections, the samples were tested by DAS-ELISA according to manufacturer’s instructions (SEDIAG, France). Qiagen clc genomics workbench 7 5 software Clc Genomics Workbench 7 5 Software, supplied by Qiagen, used in various techniques.
7.5.1 software (CLC Bio, Qiagen, Manchester, UK). Therefore, a survey was carried out during August 2019, a total of 45 leaf samples showing virus-like symptoms were randomly collected from 12 squash fields in Aksaray province in the Central Anatolian Region of Turkey. Quality-trimmed FASTQ files for each sample were imported into the CLC Genomics Workbench. Clc Genomics Workbench 7 5 1 Software, supplied by Qiagen, used in various techniques. 2018), the possibility of transmission of the virus was very high by imported plant material to Turkey. Since, Moroccan watermelon mosaic virus (MWMV, Genus: Potyvirus) has already been reported several countries in the Mediterranean basin (Bananej et al. Recently, virus diseases have become one of the most common and devastating problems in cultivation of edible seed squash ( C. Squash ( Cucurbita pepo L.) is grown for fresh consumption and its seeds are used as a snack in Turkey like in some Mediterranean and European countries, and China.
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